Genetic identification of bucktooth parrotfish Sparisoma radians (Valenciennes, 1840) (Labridae, Scarinae) by chromosomal and molecular markers

Genetics and Molecular Biology

Fabilene Gomes Paim²  José Henrique Souza Galdino Brandão²  Iracilda Sampaio¹  Paulo Roberto Antunes De Mello Affonso²  Débora Diniz² 

[1] ,Universidade Estadual do Sudoeste da Bahia Departamento de Ciências Biológicas Jequié BA ,Brazil

关键词: COI;    karyotype;    South Atlantic fish;    ribossomal genes;    Robertsonian rearrangements;   

DOI  :  10.1590/S1415-47572014005000024

来源: SciELO

【 摘 要 】

Parrotfishes (Labridae, Scarinae) comprise a large marine fish group of difficult identification, particularly during juvenile phase when the typical morphology and coloration of adults are absent. Therefore, the goal of this study was to test cytogenetic markers and DNA barcoding in the identification of bucktooth parrtotfish Sparisoma radians from the northeastern coast of Brazil. Sequencing of cytochrome c oxidase subunit I (COI) confirmed all studied samples as S. radians, and all showed high similarity (99-100%) with Caribbean populations. The karyotype of this species was divergent from most marine Perciformes, being composed of 2n = 46 chromosomes. These consisted of a large number of metacentric and submetacentric pairs with small amounts of heterochromatin and GC-rich single nucleolar organizer regions (NORs) not syntenic to 5S rDNA clusters. These are the first data about DNA barcoding in parrotfish from the Brazilian province and the first refined chromosomal analysis in Scarinae, providing useful data to a reliable genetic identification of S. radians.

【 授权许可】

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