期刊论文详细信息
Genetics and Molecular Biology
Spermatogenesis and nucleolar activity in Triatoma klugi (triatomine, Heteroptera)
Laiana Cristina Da Costa1  Maria Tercília Vilela De Azeredo-oliveira1  Ester Tartarotti1 
[1],Universidade Estadual Paulista ‘Julio de Mesquita Filho’ Instituto de Biociências, Letras e Ciências Exatas Departamento de BiologiaSão José do Rio Preto SP ,Brazil
关键词: Triatoma klugi;    Triatomine;    cytogenetics;    nucleolus;    karyotype;    meiosis;   
DOI  :  10.1590/S1415-47572008000300008
来源: SciELO
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【 摘 要 】
Triatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugi and a meiotic analysis including the nucleolar behavior during spermatogenesis. Testis cells were analyzed after lacto-acetic orcein and silver nitrate staining. Two autosomes and the heterochromosomes presented nucleolar activity (Ag-NORs) during diplotene-diakinesis. The analysis of metaphase I and II revealed a karyotype with 2n = 20+XY. In metaphase I a prominent nucleolar mass was observed in the cell periphery and small silver grains were detected in metaphase II. During anaphase, the chromosomes segregated in parallel and a typical holocentric late migration behavior was observed. The restoration of the nucleolus was an important feature in this phase. During telophase nucleolar masses persisted and in early spermiogenesis the spermatids presented a small peripheral mass until elongation. The present study is a contribution to the study of chromatin behavior and nucleolar persistence in meiosis.
【 授权许可】

CC BY   
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