Non-invasive genetic sampling for molecular sexing and microsatellite genotyping of hyacinth macaw (Anodorhynchus hyacinthinus)

Genetics and Molecular Biology

Flavia T. Presti²  Janaína Meyer²  Paulo T.z. Antas¹  Neiva M.r. Guedes¹  Cristina Y. Miyaki² 

[1] ,Universidade de São Paulo Instituto de Biociências Departamento de Genética e Biologia EvolutivaSão Paulo SP ,Brazil

关键词: molted feathers;    blood sample;    DNA;    PCR product;    parrot;   

DOI  :  10.1590/S1415-47572013005000001

来源: SciELO

【 摘 要 】

Molted feather sampling is a useful tool for genetic analyses of endangered species, but it is often very laborious due to the low quality and quantity of the DNA obtained. In the present study we show the parts of feathers that resulted in better yield of DNA. In descending order these were: blood clot outside the umbilicus, umbilicus (without blood clot), tip, inner membrane, and small calamus. Compared to DNA extracted from blood samples, DNA extracted from feathers produced microsatellite alleles of poorer quality and had to be processed immediately after extraction. As expected due to the level of DNA degradation, molecular sexing protocols that result in shorter PCR products were more efficient.

【 授权许可】

CC BY   
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