Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae)

Genetics and Molecular Biology

Marília De França Rocha²  Natoniel Franklin De Melo¹  Maria José De Souza¹ 

[1] ,Universidade de Pernambuco Instituto de Ciências Biológicas Laboratório de Biodiversidade e Genética de InsetosRecife PE ,Brazil

关键词: constitutive heterochromatin;    fluorochromes;    FISH;    Orthoptera;    ribosomal DNA;   

DOI  :  10.1590/S1415-47572011000200008

来源: SciELO

【 摘 要 】

The grasshopper species Orthoscapheus rufipes and Eujivarus fusiformis were analyzed using several cytogenetic techniques. The karyotype of O. rufipes, described here for the first time, had a diploid number of 2n = 23, whereas E. fusiformis had a karyotype with 2n = 21. The two species showed the same mechanism of sex determination (XO type) but differed in chromosome morphology. Pericentromeric blocks of constitutive heterochromatin (CH) were detected in the chromosome complement of both species. CMA3/DA/DAPI staining revealed CMA3-positive blocks in CH regions in four autosomal bivalents of O. rufipes and in two of E. fusiformis. The location of active NORs differed between the two species, occurring in bivalents M6 and S9 of O. rufipes and M6 and M7 of E. fusiformsi. The rDNA sites revealed by FISH coincided with the number and position of the active NORs detected by AgNO3 staining. The variability in chromosomal markers accounted for the karyotype differentiation observed in the tribe Abracrini.

【 授权许可】

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