Efficiency of IRAP and ITS-RFLP marker systems in accessing genetic variation of Pyrenophora graminea

Genetics and Molecular Biology

Imad Zein¹  Mohammed Jawhar¹  Mohammed Imad Eddin Arabi¹ 

[1] ,AECS Department of Molecular Biology and Microbiology ,Syria

关键词: Pyrenophora graminea;    IRAP;    internal transcribed spacer;    rDNA;   

DOI  :  10.1590/S1415-47572010005000041

来源: SciELO

【 摘 要 】

The usefulness of IRAP (inter-retrotransposon amplified polymorphism) and ITS-RFLP (restriction of PCR-amplified internal transcribed spacers of the rDNA) markers in the analysis of 39 Pyrenophora graminea isolates was determined. Each marker system could discriminate between all of the isolates in detecting polymorphism, albeit with variable efficiency. IRAP and ITS-RFLP produced 85% and 77% polymorphic bands, respectively, with a corresponding mean polymorphic information content (PIC) of 0.38 and 0.36. The IRAP marker index ratio (2.41) was higher than ITS-RFLP (1.50). On one hand, the quality nature of data (QND) was higher for ITS-RFLP (0.169) than IRAP (0.093). However, correlation between both marker similarity matrices was significant (r = 0.34, p < 0.05). These findings suggest their combined use in phylogenetic analysis. To our knowledge, this is the first report of a comparison involving these two advanced DNA marker systems.

【 授权许可】

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