Restriction analysis of the ITS region for characterization of the Debaryomyces species

The Journal of General and Applied Microbiology

Allen N. Hagler²  Orilio Leoncini¹  Patricia Valente²  Jesus P. Ramos¹ 

[1]Departamento de Genética, Instituto de Biologia, CCS, bloco A, Universidade Federal do Rio de Janeiro

[2]Instituto de Microbiologia Prof. Paulo de Goes, Universidade Federal do Rio de Janeiro

关键词: Candida;    Debaryomyces;    internal transcribed spacer;    rDNA;    yeast taxonomy;   

DOI  :  10.2323/jgam.44.399

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

The internal transcribed spacer (ITS) region of rDNA was used for taxonomic inferences in ascomycetous yeasts. The Debaryomyces species had a 640–690 ITS size. The analyzed Candida species can be differentiated by its distinct ITS size. The enzymatic digestion of the ITS region show large homogeneity in Debaryomyces, with polymorphism for only two enzymes. The ITS size and the enzymatic restriction method were used in Brazilian isolates, detecting some Debaryomyces misidentifications in cultures previously identified by conventional methods.

【 授权许可】

Unknown