Genotoxic evaluation of an industrial effluent from an oil refinery using plant and animal bioassays

Genetics and Molecular Biology

Fernando Postalli Rodrigues²  José Pedro Friedmann Angeli²  Mário Sérgio Mantovani²  Carmen Luisa Barbosa Guedes¹  Berenice Quinzani Jordão² 

[1] ,Universidade Estadual de Londrina Centro de Ciências Biológicas Departamento de Biologia GeralLondrina PR ,Brazil

关键词: Allium cepa;    HTC cells;    micronucleus;    comet assay;    petroleum waste disposal;   

DOI  :  10.1590/S1415-47572010005000006

来源: SciELO

【 摘 要 】

Polycyclic aromatic hydrocarbons (PAHs) are genotoxic chemicals commonly found in effluents from oil refineries. Bioassays using plants and cells cultures can be employed for assessing environmental safety and potential genotoxicity. In this study, the genotoxic potential of an oil refinery effluent was analyzed by means of micronucleus (MN) testing of Alium cepa, which revealed no effect after 24 h of treatment. On the other hand, primary lesions in the DNA of rat (Rattus norvegicus) hepatoma cells (HTC) were observed through comet assaying after only 2 h of exposure. On considering the capacity to detect DNA damage of a different nature and of these cells to metabolize xenobiotics, we suggest the association of the two bioassays with these cell types, plant (Allium cepa) and mammal (HTC) cells, for more accurately assessing genotoxicity in environmental samples.

【 授权许可】

CC BY   
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