Sewage sludge does not induce genotoxicity and carcinogenesis

Genetics and Molecular Biology

Paula Regina Pereira Silva²  Luis Fernando Barbisan¹  Maria Lúcia Zaidan Dagli¹  Paulo Hilário Nascimento Saldiva¹ 

[1] ,Universidade Estadual Paulista Faculdade de Medicina Departamento de Patologia, Núcleo de Avaliação do Impacto Ambiental sobre a Saúde HumanaBotocatu SP ,Brazil

关键词: comet assay;    micronucleus;    sewage genotoxicity;   

DOI  :  10.1590/S1415-47572012005000054

来源: SciELO

【 摘 要 】

Through a series of experiments, the genotoxic/mutagenic and carcinogenic potential of sewage sludge was assessed. Male Wistar rats were randomly assigned to four groups: Group 1 - negative control; Group 2 - liver carcinogenesis initiated by diethylnitrosamine (DEN; 200 mg/kg i.p.); Group 3 and G4- liver carcinogenesis initiated by DEN and fed 10,000 ppm or 50,000 ppm of sewage sludge. The animals were submitted to a 70% partial hepatectomy at the 3rd week. Livers were processed for routine histological analysis and immunohistochemistry, in order to detect glutathione S-transferase positive altered hepatocyte foci (GST-P+ AHF). Peripheral blood samples for the comet assay were obtained from the periorbital plexus immediately prior to sacrificing. Polychromatic erythrocytes (PCEs) were analyzed in femoral bone-marrow smears, and the frequencies of those micronucleated (MNPCEs) registered. There was no sewage-sludge-induced increase in frequency of either DNA damage in peripheral blood leucocytes, or MNPCEs in the femoral bone marrow. Also, there was no increase in the levels of DNA damage, in the frequency of MNPCEs, and in the development of GST-P AHF when compared with the respective control group.

【 授权许可】

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