Discrimination of Shark species by simple PCR of 5S rDNA repeats

Genetics and Molecular Biology

Danillo Pinhal²  Otto Bf Gadig¹  Adriane P Wasko¹  Claudio Oliveira²  Ernesto Ron¹  Fausto Foresti²  Cesar Martins² 

[1] ,Universidade Estadual Paulista Instituto de Biociências Departamento de MorfologiaBotucatu SP ,Brazil

关键词: Chondrichthyes;    PCR;    species identification;    5S rDNA;    sharks;   

DOI  :  10.1590/S1415-47572008000200033

来源: SciELO

【 摘 要 】

Sharks are suffering from intensive exploitation by worldwide fisheries leading to a severe decline in several populations in the last decades. The lack of biological data on a species-specific basis, associated with a k-strategist life history make it difficult to correctly manage and conserve these animals. The aim of the present study was to develop a DNA-based procedure to discriminate shark species by means of a rapid, low cost and easily applicable PCR analysis based on 5S rDNA repeat units amplification, in order to contribute conservation management of these animals. The generated agarose electrophoresis band patterns allowed to unequivocally distinguish eight shark species. The data showed for the first time that a simple PCR is able to discriminate elasmobranch species. The described 5S rDNA PCR approach generated species-specific genetic markers that should find broad application in fishery management and trade of sharks and their subproducts.

【 授权许可】

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