Over-activation of the Drosophila melanogaster hsp83 gene by selenium intoxication

Genetics and Molecular Biology

Ronaldo Medeiros Golombieski²  Daniel Ângelo Sgranzerla Graichen¹  João Batista Teixeira Da Rocha²  Vera Lúcia Da Silva Valente¹  Élgion Lúcio Da Silva Loreto¹ 

[1] ,Universidade Federal de Santa MariaSanta Maria RS ,Brazil

关键词: gene activation;    hsp83 gene;    selenium;    diphenyl diselenide;   

DOI  :  10.1590/S1415-47572008000100022

来源: SciELO

【 摘 要 】

Selenium is an important dietary micronutrient and an essential component of selenoproteins and the active sites of some enzymes, although at high concentrations it is toxic. We investigated diphenyl diselenide ((C6H5)2Se2 ) for its effects on the developmental stages of Drosophila melanogaster and found that in the larval and pupae stages the toxic threshold for this compound when added to the banana-agar medium on which the larva were fed was 350 µmol. In adult flies, fed on the same media, there were no observable toxic effects below 500 µmol but there were toxic effects above 600 µmol, indicating that adult flies were more resistant to selenium intoxication. In larvae, a when diphenyl diselenide was present above the toxic threshold there was increased activation of the hsp83 heat shock protein gene. Selenium promotes oxidation of sulfhydryl groups and affects the folding of proteins and this could explain the over-expression of hsp83 because the product of this gene is involved in protein folding and defense responses, including the response to heat shock.

【 授权许可】

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