Differential expression of genes identified from Poncirus trifoliata tissue inoculated with CTV through EST analysis and in silico hybridization

Genetics and Molecular Biology

Mariângela Cristofani-yaly¹  Irving J. Berger¹  Maria Luisa P.n. Targon¹  Marco A. Takita¹  Sílvia De O. Dorta¹  Juliana Freitas-astúa¹  Alessandra A. De Souza¹  Raquel L. Boscariol-camargo¹  Marcelo S. Reis¹  Marcos A. Machado¹ 

[1] ,Instituto Agronômico de Campinas Centro APTA Citros Sylvio Moreira Laboratório de BiotecnologiaCordeirópolis SP ,Brazil

关键词: citrus;    disease resistance;    Citrus tristeza virus;    biotic stress;   

DOI  :  10.1590/S1415-47572007000500025

来源: SciELO

【 摘 要 】

Citrus is the most important fruit crop in Brazil and Citrus tristeza virus (CTV) is considered one of the most important pathogens of citrus. Most citrus species and varieties are susceptible to CTV infection. However, Poncirus trifoliata, a close relative of citrus, is resistant to the virus. In order to better understand the responses of citrus plants to the infection of CTV, we constructed expressed sequence tag (EST) libraries with tissues collected from Poncirus trifoliata plants, inoculated or not with Citrus tristeza virus at 90 days after inoculation, grafted on Rangpur lime rootstocks. We generated 17,867 sequence tags from Poncirus trifoliata inoculated (8,926 reads) and not (8,941 reads) with a severe CTV isolate. A total of 2,782 TCs (Tentative Consensi sequences) were obtained using both cDNA libraries in a single clusterization procedure. By the in silico hybridization approach, 289 TCs were identified as differentially expressed in the two libraries. A total of 121 TCs were found to be overexpressed in plants infected with CTV and were grouped in 12 primary functional categories. The majority of them were associated with metabolism and defense response. Some others were related to lignin, ethylene biosynthesis and PR proteins. In general, the differentially expressed transcripts seem to be somehow involved in secondary plant response to CTV infection.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License