Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil

Brazilian Journal of Infectious Diseases

Ana Claudia Souza Rodrigues²  Marilene Rodrigues Chang¹  Gabriela Dorn Nóbrega¹  Mayara Souza Rodrigues¹  Nadia Cristina Pereira Carvalho¹  Beatriz Garcia Gomes¹  Dênio Lopes De Almeida¹  Karyne Rangel Carvalho¹  Marise Dutra Asensi¹ 

[1] ,Universidade Federal de Mato Grosso do Sul Health and Development Post-graduation Program, Central-West RegionMS ,Brazil

关键词: Pseudomonas aeruginosa;    drug resistance;    multiple;    beta-lactamases;    electrophoresis;    gel;    pulsed-field;   

DOI  :  10.1590/S1413-86702011000300002

来源: SciELO

【 摘 要 】

Infection by Pseudomonas aeruginosa has spread worldwide, with limited options for treatment. The purpose of this study was to investigate metallo-β-lactamase-producing P. aeruginosa strains and compare their genetic profile using samples collected from patients in intensive care units. Forty P. aeruginosa strains were isolated from two public hospitals in Campo Grande, Mato Grosso do Sul State, from January 1st, 2007 to June 31st, 2008. Profiles of antimicrobial susceptibility were determined using the agar diffusion method. Metallo-β-lactamase was investigated using the double-disk diffusion test and PCR. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE). Respiratory and urinary tracts were the most common isolation sites. Of the 40 samples tested, 72.5% (29/40) were resistant to ceftazidime and 92.5% (37/40) to imipenem, whereas 65% (26/40) were resistant to both antimicrobials. Fifteen pan-resistant samples were found. Five percent (2/40) of samples were positive for metallo-β-lactamase on the phenotype test. No metallo-β-lactamase subtype was detected by PCR. Macrorestriction analysis revealed 14 distinct genetic patterns. Based on the superior accuracy of PCR, it can be inferred that P. aeruginosa isolates from the investigated hospitals have alternative mechanisms of carbapenem resistance. The results also suggest clonal spread of P. aeruginosa between the studied hospitals.

【 授权许可】

CC BY-NC-ND   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License