Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil

Brazilian Journal of Infectious Diseases

Chang, Marilene Rodrigues²  Almeida, Dênio Lopes de²  Carvalho, Karyne Rangel³  Nóbrega, Gabriela Dorn²  Rodrigues, Ana Claudia Souza²  Rodrigues, Mayara Souza²  Carvalho, Nadia Cristina Pereira²  Gomes, Beatriz Garcia¹  Asensi, Marise Dutra³ 

[1] Hospital Regional de Mato Grosso do Sul, Brazil;Universidade Federal de Mato Grosso do Sul, Brazil;FIOCRUZ, Brazil

关键词: Pseudomonas aeruginosa;    drug resistance;    multiple;    beta-lactamases;    electrophoresis;    gel;    pulsed-field.;   

DOI  :  10.1590/S1413-86702011000300002

来源: Contexto

【 摘 要 】

Infection by Pseudomonas aeruginosa has spread worldwide, with limited options for treatment. The purpose of this study was to investigate metallo-β-lactamase-producing P. aeruginosa strains and compare their genetic profile using samples collected from patients in intensive care units. Forty P. aeruginosa strains were isolated from two public hospitals in Campo Grande, Mato Grosso do Sul State, from January 1^st, 2007 to June 31^st, 2008. Profiles of antimicrobial susceptibility were determined using the agar diffusion method. Metallo-β-lactamase was investigated using the double-disk diffusion test and PCR. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE). Respiratory and urinary tracts were the most common isolation sites. Of the 40 samples tested, 72.5% (29/40) were resistant to ceftazidime and 92.5% (37/40) to imipenem, whereas 65% (26/40) were resistant to both antimicrobials. Fifteen pan-resistant samples were found. Five percent (2/40) of samples were positive for metallo-β-lactamase on the phenotype test. No metallo-β-lactamase subtype was detected by PCR. Macrorestriction analysis revealed 14 distinct genetic patterns. Based on the superior accuracy of PCR, it can be inferred that P. aeruginosa isolates from the investigated hospitals have alternative mechanisms of carbapenem resistance. The results also suggest clonal spread of P. aeruginosa between the studied hospitals.

【 授权许可】

CC BY-NC-ND