Enrichment methodology to increase the positivity of cultures from body fluids

Brazilian Journal of Infectious Diseases

Alessandra Valle Daur¹  Francisco Klimak Jr.¹  Laura Lúcia Cogo¹  Gislene Diógenes Botão¹  Cristina Leise Bastos Monteiro¹  Libera Maria Dalla Costa¹ 

[1] ,Curitiba General Hospital Clinical Laboratory

关键词: Body fluids;    etiological agents;    enrichment;   

DOI  :  10.1590/S1413-86702006000600002

来源: SciELO

【 摘 要 】

Isolation and identification of etiological agents found in body fluids can be of critical importance for the recovery of patients suffering from potentially-severe infections, which are often followed by serious sequels. Eighty-two samples of different body fluids were analyzed using two different methods: (1) the conventional culture method (agar plating) and (2) the enrichment culture technique, using the Bact/Alert® blood culture bottle. The number of positive cultures increased on average from 9.7% to 23.1% with the enrichment culture technique. Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus were the most frequently isolated bacteria. The enrichment method could provide a more accurate means the identifying etiological agents.

【 授权许可】

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