Identification of haploid maize by flow cytometry, morphological and molecular markers

Ciência e Agrotecnologia

Evellyn Giselly De Oliveira Couto²  Livia Maria Chamma Davide¹  Fernanda De Oliveira Bustamante¹  Renzo Garcia Von Pinho¹  Tallyta Nayara Silva¹ 

[1] ,Universidade Federal de LavrasLavras MG ,Brasil

关键词: Zea mays;    nuclear DNA content;    androgenetic inheritance;    R-navajo;    microsatellites;    Zea mays;    conteúdo de DNA nuclear;    herança androgenética;    R-navajo;    microssatélites;   

DOI  :  10.1590/S1413-70542013000100003

来源: SciELO

【 摘 要 】

The development of homozygous breeding lines in maize may be accelerated through the use of haploids. Thus, the obtaining and prior identification of haploids generated by the haploid inducer lines is an important factor. The purpose of this study was to identify haploids by flow cytometry and to correlate the nuclear DNA content to the morphological and morphometric traits of the seeds that gave rise to them. In addition, molecular markers were used to confirm the androgenetic nature of the haploid. The seeds obtained were derived from the cross between the inbred line W23 and the commercial hybrid P30F90. Among these seeds, a group was selected, putative haploids, whose embryo was white and the pericarp purplish. This group, consisting of 330 seeds, was characterized based on seed morphology, seed morphometry and nuclear DNA content. Flow cytometry analyses identified four haploids, and all of them were small size plants and had brittle leaves. The weight, length, thickness and width of the haploid seeds were very variable indicating that morphometric traits do not constitute reliable data for visual selection of haploid seeds. Based on results, the inbred line W23 induced haploid maize even under tropical conditions. Microsatellite molecular markers (SSR) proved to be efficient, confirming the androgenetic trait of the haploids.

【 授权许可】

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