In vivo HAPLOID INDUCTION AND EFFICIENCY OF TWO CHROMOSOME DUPLICATION PROTOCOLS IN TROPICAL MAIZE

Ciência e Agrotecnologia

Evellyn Giselly De Oliveira Couto¹  Édila Vilela De Resende Von Pinho¹  Renzo Garcia Von Pinho¹  Adriano Delly Veiga¹  Fernanda De Oliveira Bustamante¹  Kaio Olimpio Das Graças Dias¹ 

关键词: R-Navajo;    colchicine;    induction of maternal haploids;    Zea mays;    R-navajo;    colchicina;    indução de haploides maternos;    Zea mays;   

DOI  :  10.1590/S1413-70542015000500002

来源: SciELO

【 摘 要 】

ABSTRACTArtificial chromosome duplication is one of the most important process in the attainment of doubled haploids in maize. This study aimed to evaluate the induction ability of the inducer line KEMS in a tropical climate and test the efficiency of the R1-Navajo marker by flow cytometry to evaluate two chromosome duplication protocols and analyze the development of the doubled haploids in the field. To accomplish this goal, four genotypes (F1 and F2 generations) were crossed with the haploid inducer line KEMS. The seeds obtained were selected using the R1-Navajo marker and subject to two chromosome duplication protocols. Duplication was confirmed using flow cytometry. The percentages of self-fertilized plants after duplication as well as the quantities of doubled haploid seeds obtained after the self-fertilization processes were analyzed. It was observed that the germplasm influences haploid induction but not the duplication rates of the tested protocols. Protocol 2 was more efficient for the duplication of haploids, in the percentage of self-fertilized plants after duplication, and in the attainment of doubled haploid lines. Moreover, the haploid inducer line KEMS can produce haploids in a tropical climate. Other markers, in addition to the R1-Navajo system, should be used in the selection of haploid seeds.

【 授权许可】

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