Bioassay method for toxicity studies of insecticide formulations to Tuta absoluta (meyrick, 1917)

Ciência e Agrotecnologia

Tarcísio Visintin Da Silva Galdino²  Marcelo Coutinho Picanço¹  Elisangela Gomes Fidelis De Morais¹  Nilson Rodrigues Silva¹  Geverson Aelton Rezende Da Silva¹  Mayara Cristina Lopes¹ 

[1] ,Universidade Federal de Viçosa Departamento de Biologia Animal Viçosa MG

关键词: Susceptibility;    tomato leafminer;    chemical control;    bioassay standardization;    resistance survey;    Suscetibilidade;    traça-do-tomateiro;    controle químico;    padronização de bioensaio;    levantamento de resistência;   

DOI  :  10.1590/S1413-70542011000500002

来源: SciELO

【 摘 要 】

Chemical control is the main method for controlling the tomato leafminer, Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae). Reported techniques for the evaluation of insecticide toxicity to the tomato leafminer are not in agreement with field conditions and do not allow us to verify whether doses used in the field are efficient for control. Thus, the objective of this work was to develop a bioassay methodology to study the toxicity of insecticide formulations to T. absoluta that represent field conditions for fast-acting insecticides (neurotoxics and inhibitors of respiration) and slow-acting insecticides (Bacillus thuringiensis and insect growth regulators). The leaf-dip method was the most efficient method for toxicity studies of insecticides formulations to T. absoluta. We verified that bioassays with fast-acting insecticides should be performed with glass Petri dishes containing one tomato foliole from the 4th leaf from the plant apex infested with 10 larvae of 3rd instar and these bioassays can last 48 hours. Conversely, bioassays with slow-acting insecticides should be performed with two-liter transparent PET bottles containing the 4th leaf from the plant apex, with their petioles immersed in a glass bottle containing 120 mL of water, and this leaf should be infested with 10 larvae of 2nd instar and this bioassays can last seven days.

【 授权许可】

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