Scanning electron microscopy of a blister roof in dystrophic epidermolysis bullosa

Anais Brasileiros de Dermatologia

Hiram Larangeira De Almeida Jr.¹  Luciane Monteiro¹  Ricardo Marques E Silva¹  Nara Moreira Rocha¹  Hans Scheffer¹ 

关键词: Basement membrane;    Epidermolysis bullosa dystrophica;    Microscopy;    electron;    scanning;    Skin diseases;    vesiculobullous;    Dermatopatias vesiculobolhosas;    Epidermólise bolhosa distrófica;    Membrana basal;    Microscopia eletrônica de varredura;   

DOI  :  10.1590/abd1806-4841.20131743

来源: SciELO

【 摘 要 】

In dystrophic epidermolysis bullosa the genetic defect of anchoring fibrils leads to cleavage beneath the basement membrane, with its consequent loss. We performed scanning electron microscopy of an inverted blister roof of a case of dystrophic epidermolysis bullosa, confirmed by immunomapping and gene sequencing. With a magnification of 2000 times a net attached to the blister roof could be easily identified. This net was composed of intertwined flat fibers. With higher magnifications, different fiber sizes could be observed, some thin fibers measuring around 80 nm and thicker ones measuring between 200 and 300 nm.

【 授权许可】

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