Performance of a biosurfactant produced by Bacillus subtilis LAMI005 on the formation of oil / biosurfactant / water emulsion: study of the phase behaviour of emulsified systems

Brazilian Journal of Chemical Engineering

M. Sousa²  I. T. Dantas²  F. X. Feitosa²  A. E. V. Alencar¹  S. A. Soares¹  V. M. M. Melo¹  L. R. B. Gonçalves²  H. B. Sant'ana² 

[1] ,Universidade Federal do Ceará Departamento de Engenharia Química Fortaleza CE ,Brasil

关键词: Biosurfactants;    Emulsions;    Phase diagrams;    Residual glycerol;    Bacillus subtilis;   

DOI  :  10.1590/0104-6632.20140313s00002766

来源: SciELO

【 摘 要 】

In this study, the phase behaviour of emulsified systems (oil + biosurfactant + water) was analyzed. The biosurfactant was produced in a 4-L batch bioreactor by Bacillus subtilis LAMI005, using residual glycerine from biodiesel production as a carbon source. Fourier Transform Infrared Spectroscopy (FT-IR), Nuclear Magnetic Resonance (NMR), and High Performance Liquid Chromatography (HPLC) analyses demonstrated that the biosurfactant produced by Bacillus subtilis (LAMI005) consists of a lipopeptide similar to surfactin. The influences of temperature and the composition of oil + biosurfactant + water were determined by using phase diagrams. Three types of oil were used, namely: motor oil, hydrogenated naphthenic oil (NH140) and castor bean oil. The emulsified systems were analyzed using optical micrography. The results presented here indicated that the biosurfactant produced in this work presents a potential use as stabilizing agent for oil-in-water emulsions.

【 授权许可】

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