Maintenance-energy-dependent dynamics of growth and poly(3-hydroxybutyrate) [P(3HB)] production by Azohydromonas lata MTCC 2311 using simple and renewable carbon substrates

Brazilian Journal of Chemical Engineering

M. Zafar²  S. Kumar¹  A. K. Dhiman¹  H.-s. Park¹ 

[1] ,Shobhit University Department of Biological Engineering U.P.,India

关键词: Poly(3-hydroxybutyrate);    Microbial dynamics;    Cane molasses;    Maintenance energy;    Azohydromonas lata;   

DOI  :  10.1590/0104-6632.20140312s00002434

来源: SciELO

【 摘 要 】

The dynamics of microbial growth and poly(3-hydroxybutyrate) [P(3HB)] production in growth/ non-growth phases of Azhohydromonas lata MTCC 2311 were studied using a maintenance-energy-dependent mathematical model. The values of calculated model kinetic parameters were: m s1 = 0.0005 h-1, k = 0.0965, µmax = 0.25 h-1 for glucose; m s1 = 0.003 h-1, k = 0.1229, µmax = 0.27 h-1 for fructose; and m s1 = 0.0076 h-1, k = 0.0694, µmax = 0.25 h-1 for sucrose. The experimental data of biomass growth, substrate consumption, and P(3HB) production on different carbon substrates were mathematically fitted using non-linear least square optimization technique and similar trends, but different levels were observed at varying initial carbon substrate concentration. Further, on the basis of substrate assimilation potential, cane molasses was used as an inexpensive and renewable carbon source for P(3HB) production. Besides, the physico-chemical, thermal, and material properties of synthesized P(3HB) were determined which reveal its suitability in various applications.

【 授权许可】

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