Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus

Brazilian Journal of Chemical Engineering

S. W. Cheng²  Y. F. Wang¹  B. Hong² 

[1] ,Ludong University School of Life Sciences Institute of Applied MicrobiologyYantai,China

关键词: Chitosanase;    Fermentation optimization;    Medium compositions;    Response surface methodology;    Streptomyces albus;   

DOI  :  10.1590/S0104-66322012000400002

来源: SciELO

【 摘 要 】

The nutritional medium compositions required for chitosanase production by Streptomyces albus YT2 were optimized, and the strain was isolated from the coastal soil of Bohai Sea. Plackett-Burman design was applied to evaluate the effect of different medium compositions obtained by one-factor-at-a-time experiments. Glucose, peptone and MgSO4 were found to influence the activity of chitosanase significantly. The steepest ascent method was employed to approach the experimental design space, followed by the use of Box-Behnken design and response surface methodology for further optimization of the chitosanase production. The medium composition for optimal production of chitosanase was as follows: 0.5 g/L chitosan, 55 g/L glucose, 1 g/L peptone, 2 g/L (NH4)2SO4, 8 g/L urea, 1 g/L NH4Cl, 3 g/L KH2PO4, 1 g/L FeSO4·7H2O, 2 g/L ZnSO4·7H2O, 2 g/L CaCl2·6H2O, 2 g/L MnSO4·H2O and 2 g/L NaCl. The chitosanase production was found to increase from 11.56 U/mL to 39.87 U/mL, a 3.47-fold increase compared with that using the original medium.

【 授权许可】

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