Production of native flagellin from Salmonella Typhimurium in a bioreactor and purification by tangential ultrafiltration

Brazilian Journal of Chemical Engineering

B. H. Oliveira²  M. R. Silva¹  C. J. M. Braga¹  L. M. Massis¹  L. C. S. Ferreira¹  M. E. Sbrogio-almeida¹  M. Takagi¹ 

[1] ,Universidade Federal do Paraná Departamento de Engenharia Química Curitiba PR ,Brasil

关键词: Flagellin;    Purification;    Tangential ultrafiltration;    Salmonella Typhimurium;   

DOI  :  10.1590/S0104-66322011000400003

来源: SciELO

【 摘 要 】

Flagellin is the structural protein and most abundant component of bacterial flagella. The flagellum filament contains around 20,000 - 100,000 subunits of 50 kDa flagellin that can have diverse biotechnological applications such as vaccine adjuvant and cellular protector during chemo- and radiotherapy. The main aim of this work was to study a production process of purified native FliC flagellin of Salmonella Typhimurium. The culture conditions in shakers were established with medium devoid of animal-derived components. In bioreactors, culture conditions were established in order to obtain flagellin from the culture supernatant by tangential ultrafiltration (TUF). The concentrated 750 kDa cut-off TUF fraction had a purification factor of 1.5 and a recovery yield of 52.2% for flagellin. The volumetric production of flagellin using the described procedure achieved around 307 mg/L of culture, which represented a significant improvement over previously reported methods. These results permit the development of production and purification processes that can be easily scaled up.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License