Liquid - liquid extraction of matrine using TRPO/cyclohexane reverse micelles

Brazilian Journal of Chemical Engineering

Lichun Dong¹  Wenping Li¹  Xiaohua Zhou¹  Qin Kang¹  Weifeng Shen¹ 

[1] ,Chongqing University College of Chemistry and Chemical Engineering ,China

关键词: Reverse micellar extraction;    Coordination interaction;    TRPO reverse micelles;    Matrine;    Oxymatrine;   

DOI  :  10.1590/S0104-66322009000200020

来源: SciELO

【 摘 要 】

Reverse micellar extraction has been widely used in the purification of biomolecules. However, reverse micelles formed by ionic surfactants can only be employed for the extraction of biomolecules that are charged in the extraction system with the electrostatic interaction between surfactants and solutes as the driving force. In this study, the extraction of matrine by using reverse micelles formed by non-ionic TRPO surfactants was studied. Theoretical analysis and experimental results demonstrated that the driving force of the extraction is the coordination forces between matrines and TRPOs. Using this coordination-based reverse micellar extraction, matrine can be efficiently separated from oxymatrine and other components in the raw matrine materials. Experimental studies showed that the factors affecting matrine extraction include pH value and TRPO concentration. The existence of ions in the system does not affect the partition coefficient significantly and the addition of a small amount of chloroform in the solution of reverse micelles was found to improve the extraction significantly.

【 授权许可】

CC BY   
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