Matrine Activates PTEN to Induce Growth Inhibition and Apoptosis in V600EBRAF Harboring Melanoma Cells

International Journal of Molecular Sciences

Hui Jin¹  Yu Sun²  Shuiying Wang² 

[1] School of Life Sciences and Technology, Tongji University, Shanghai 200092, China; E-Mail:;Yue-yang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China; E-Mails:

关键词: Matrine;    cell cycle arrest;    apoptosis;    ^V600EBRAF melanoma;    PTEN;   

DOI  :  10.3390/ijms140816040

来源: mdpi

【 摘 要 】

Here, we report a natural chemical Matrine, which exhibits anti-melanoma potential with its PTEN activation mechanism. Matrine effectively inhibited proliferation of several carcinoma cell lines, including melanoma ^V600EBRAF harboring M21 cells. Flow cytometry analysis showed Matrine induced G₀/G₁ cell cycle arrest in M21 cells dose-dependently. Apoptosis in M21 cells induced by Matrine was identified by Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) analysis and Annexin-V/FITC staining. Molecular mechanistic study suggested that Matrine upregulated both mRNA level and protein expression level of phosphatase and tensin homolog deleted on chromosome ten (PTEN), leading to inhibition of the PI3K/Akt pathway. Downregulation of phosphor-Akt^ser473 by Matrine activated p21 and Bax, which contributed to G₀/G₁ cell cycle and apoptosis. Besides, Matrine enhanced the PI3K/Akt inhibition effects to inhibit the cell proliferation with PI3K inhibitor, LY2940002. In summary, our findings suggest Matrine is a promising antitumor drug candidate with its possible PTEN activation mechanisms for treating cancer diseases, such as melanomas.

【 授权许可】

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© 2013 by the authors; licensee MDPI, Basel, Switzerland