Survival and mutant production induced by mutagenic agents in Metarhizium anisopliae

Scientia Agricola

V. Kava - Cordeiro²  E.a. Luna - Alves - Lima¹  J.l. Azevedo¹ 

[1] ,VFPR Depto. Genética Curitiba PR

关键词: Metarhizium anisopliae;    auxotrophk mutants;    colour mutants;    mutagenic agents;    gamma radiation;    ultraviolet light;    nitrous acid;    Metarhizium anisopliae;    agentes mutagênicos;    mutantes auxotróficos;    mutantes para coloração;    radiação gama;    luz ultravioleta;    ácido nitroso;   

DOI  :  10.1590/S0103-90161995000300023

来源: SciELO

【 摘 要 】

A wild strain of Metarhizium anisopliae, an entomopathogenic fungus, was submitted to three mutagenic agents: gamma radiation, ultraviolet light and nitrous acid. Survival curves were obtained and mutants were selected using different mutagenic doses which gave 1 to 5% survival. Morphological and auxotrophic mutants were isolated. Morphological mutants were grouped in a class with yellow conidia and other with pale vinaceous conidia as opposed to the green wild type conidia. Auxotrophic mutants had requirements for vitamin and aminoacid biosynthesis. More than 58% of the total auxotrophk mutants required proline/aipnine. Gamma radiation showed to be the most efficient mutagenic agent giving 0.2% of auxotrophk mutants followed by ultraviolet light (0.12%) and nitrous acid (0.06%).The conidial colour and auxotrophk mutants isolated until now from M. anisopliae were reviewed.

【 授权许可】

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