Antibacterial activity tannin-rich fraction from leaves of Anacardium humile

Ciência Rural

Peracio Rafael Bueno Ferreira²  Cíntia Sorandra Oliveira Mendes¹  Cínthia Gracielly Rodrigues¹  Juliane Caroline Moura Rocha¹  Vanessa De Andrade Royo¹  Henrique Maia Valério¹  Dario Alves De Oliveira¹ 

[1] ,Universidade Federal de Viçosa (UFV) Departamento de Química Viçosa MG ,Brasil

关键词: phenolic;    cajuí;    antibacterial;    MIC;    MBC;    Sephadex LH-20;    fenólicos;    cajuí;    antibacterianos;    CIM;    CBM;    Sephadex LH-20;   

DOI  :  10.1590/S0103-84782012005000080

来源: SciELO

【 摘 要 】

In vitro tests conducted with extracts rich in tannins have identified several biological activities of this class of substance. Thus, this paper intends to evaluate the antibacterial activity of tannin-rich fraction obtained from leaf extracts of Anacardium humile A.St.-Hil. Extracts of A. humile leaves in 70% acetone were semi-purified with ethyl acetate and butanol. We quantified the total tannins of the semi-purified fractions, of the crude extract and of aqueous residues and then performed tests of the antibacterial activity of the tannins against Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 29212. All positive extracts underwent tannin isolation using a Sephadex LH-20 column. The tannins isolated from the samples were quantified and tested for the minimal inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC). The tannins from crude extracts, semi-purifications and residues showed inhibition of S. aureus growth with MIC=500 g mL-1. All tannin fractions showed MIC against all strains and MBC, except against E. faecalis. The tannin fractions from Anacardium humile have antibacterial activity against Staphylococcus aureus, Pseudomononas aeruginosa and Enterococcus faecalis and, therefore, may be promising for future synthesis of new antibacterial agents.

【 授权许可】

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