Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development

Ciência Rural

Camila Argenta Fante²  Patrícia De Fátima Pereira Goulart¹  José Donizeti Alves¹  Paôla De Castro Henrique¹  Daniela Deitos Fries¹ 

[1] ,Universidade Federal de Lavras Departamento de Ciência dos Alimentos Lavras MG ,Brasil

关键词: Glycine max;    hypoxia;    functional food;    Glycine max;    hipoxia;    alimento funcional;   

DOI  :  10.1590/S0103-84782011001200029

来源: SciELO

【 摘 要 】

The stress imposed on plants by soil flooding constitutes a major barrier to growth and productivity. The identification of soybean varieties that produce higher levels of isoflavones, is necessary as soybeans have been used as human food to reduce risks of chronic diseases. Thus, this study was conducted with the objective of quantifying proteins and isoflavones in soybean cultivars subjected to flooding at various stages of development. The cultivars 'BRS267', 'BRS257' and 'BRS213' were subjected to 15 days of flooding, starting at the stages V6 and V8 and 11 days under stress starting at the stage R4. The proteins in the grain were extracted and quantified and analyzed by SDS-PAGE electrophoresis. Isoflavones were extracted, separated and quantified on HPLC. The electrophoretic analysis of the three cultivars under study revealed the same pattern of banding relative to the total protein regardless of the treatment. However, it was noted that flooding led to an increase in the total contents of isoflavones in the BRS 267 plants flooded in stage R4, remaining constant in other cultivars.

【 授权许可】

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