Protein and antigen profiles of Leptospira interrogans serovar Hardjo

Ciência Rural

Bárbara Nobre Lafetá¹  Elaine Cristina De Castro¹  Nivaldo Da Silva¹ 

[1] ,Universidade Federal de Minas Gerais Escola de Veterinária Departamento de Medicina Veterinária PreventivaBelo Horizonte MG ,Brasil

关键词: Leptospira interrogans;    outer membrane protein;    hardjoprajitno;    immunomodulatory proteins;    Leptospira interrogans;    proteína de membrana externa;    hardjoprajitno;    proteínas imunomodulatórias;   

DOI  :  10.1590/S0103-84782009000900024

来源: SciELO

【 摘 要 】

The protein profile of the outer membrane of Leptospira interrogans serovar Hardjo subtype hardjoprajitno associated with the bovine natural immune response was investigated. The outer membrane proteins were extracted utilizing Triton X114 and precipitated with acetone. The protein sample was then resolved by SDS-PAGE and reacted in western blot against sera from a hyperimmune rabbit and from naturally infected bovines. In silver stained gels, 14 protein bands were observed, among which four proteins, with 22, 29, 47 and 63kDa, appeared as major constituents. Western blot tests with hyperimmune rabbit antiserum detected bands corresponding to proteins with 35; 27; 24; 21; 17 and 14kDa, while 32kDa and 45kDa proteins were the most immunoreactive with sera from naturally infected bovines.

【 授权许可】

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