Autologous bone marrow transplantation in a dog with lymphoma: a clinical study

Ciência Rural

Maria Luísa Buffo De Cápua²  Flávia Eiras Dela Coleta²  Mariana Rodrigues Miotto²  Sabryna Gouveia Calazans²  Aline Vieira Godoy²  Ana Paula Massae Nakage¹  Carolina Bonduki Sales Lisbão¹  Carlos Roberto Daleck¹  Aureo Evangelista Santana¹ 

[1],Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento Clínica e Cirugia VeterináriaJaboticabal SP ,Brasil

关键词: canine;    cutaneous lymphoma;    autologous bone marrow transplant;    chemotherapy;    canino;    linfoma cutâneo;    transplante autólogo de medula óssea;    quimioterapia;   

DOI  :  10.1590/S0103-84782008005000087

来源: SciELO

【 摘 要 】

The objective of this study is to provide the first report of bone marrow transplantation (BMT) in dogs in Brazil. A Rottweiler with cutaneous lymphoma was submitted to a twelve-week Madison-Wisconsin chemotherapy protocol followed by autologous bone marrow transplantation. For this, 10mL kg-1 of bone marrow was collected simultaneously from both iliac crests and cryopreserved in a freezer at -80°C. The conditioning step was performed by administering cyclophosphamide by intravenous route at 400mg m-2. Bone marrow was reinfused after defrosting in a water bath at 37°C. Bone marrow nucleated cell counts before and after freezing, showed a small relative loss of nucleated cells (35.10 and 31.80x10³µL-1 , respectively). Cyclophosphamide induced neutropenia which was reverted by a granulocyte colony-stimulating factor (G-CSF) capable of stimulating hematopoetic reconstitution. On the day 360 after transplant the patient was found to be in complete remission. This study indicates that autologous BMT in a dog with lymphoma submitted to myelosuppressive chemotherapy was potentially safe and effective.

【 授权许可】

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