Presence of multiple acid phosphatases activity in seedlings of cucumber, radish and rocket salad

Ciência Rural

Luciane Almeri Tabaldi¹  Raquel Ruppenthal¹  Luciane Belmonte Pereira¹  Denise Cargnelutti¹  Jamile Fabbrin Gonçalves¹  Vera Maria Morsch¹  Maria Rosa Chitolina Schetinger¹ 

[1] ,Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas Departamento de QuímicaSanta Maria RS ,Brasil

关键词: Cucumis sativus;    Raphanus sativus;    Eruca vesicaria;    phosphatases;    Cucumis sativus;    Raphanus sativus;    Eruca vesicaria;    fosfatases;   

DOI  :  10.1590/S0103-84782008000300009

来源: SciELO

【 摘 要 】

Acid phosphatases (3.1.3.2) are a group of enzymes widely distributed in nature, which catalyze the hydrolysis of a variety of phosphate esters in the pH range of 4-6. We confirmed the presence of acid phosphatases in seedlings of cucumber (Cucumis sativus), radish (Raphanus sativus) and rocket salad (Eruca vesicaria) under different assay conditions using a rapid and simple preparation. The results showed that the optimum pH and temperature used for all species were close to 5.5 and 35°C, respectively. The enzyme was inhibited by molybdate, fluoride, azide, levamisole, orthovanadate, Zn2+ and Cu2+. Suramin had no effect on enzyme activity. The acid phosphatase from cucumber, radish and rocket salad hydrolyzed a wide variety of phosphate esters and the highest activity was observed with PPi, ATP and GTP. These results demonstrate that the enzyme investigated in this study is different from well known ester phosphate cleaving plant enzymes (apyrase and inorganic pyrophosphatases) and this preparation could be a useful tool to future toxicological studies and to study initially all isoforms of acid phosphatase.

【 授权许可】

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