Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, Brazil

Ciência Rural

Cleverson Busso²  Edilson Nobuyoshi Kaneshima¹  Francisco De Assis Franco¹  Carmen Boto Querol²  Marialba Avezum Alves De Castro-prado² 

[1] ,Universidade Estadual de Maringá Departamento de Biologia Celular e Genética Maringá PR ,Brasil

关键词: Fusarium graminearum;    genetic variability;    RAPD;    vegetative compatibility groups;    Fusarium graminearum;    grupos de compatibilidade vegetativa;    RAPD;    variabilidade genética;   

DOI  :  10.1590/S0103-84782007000600050

来源: SciELO

【 摘 要 】

Fusarium graminearum isolates causing Fusarium head blight in wheat were collected in Brazil and analyzed by random amplified polymorphic DNA (RAPD) markers and vegetative compatibility grouping (VCG). Nitrate non-utilizing mutants (nit) from each isolate were paired to verify heterokaryon formation. Three VCGs were identified among F. graminearum isolates: VCG1 included F-2, F-3 and F-4 isolates; VCG2 included F-1, F-6 and F-9 isolates; VCG3 included F-5, F-7 and F-8 isolates. Based on PCR amplification with eight different primers, the isolates showed great genetic similarity among themselves. Dendrogram analysis demonstrated two RAPD groups: Group A, consisting of isolates F-2 and F-9, and Group B, composed of the remaining isolates. Results suggest the clonal origin of F. graminearum isolates.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License