Genetic variability in the endophytic fungus Guignardia citricarpa isolated from citrus plants

Genetics and Molecular Biology

Chirlei Glienke-blanco¹  Carlos Ivan Aguilar-vildoso¹  Maria Lúcia Carneiro Vieira¹  Paulo Augusto Vianna Barroso¹  João Lúcio Azevedo¹ 

[1] ,Universidade de São Paulo Escola Superior de Agricultura 'Luiz de Queiroz' Department of GeneticsPiracicaba São Paulo ,Brazil

关键词: citrus;    endophytes;    genetic variability;    Guignardia citricarpa;    mandarin;    RAPD;   

DOI  :  10.1590/S1415-47572002000200021

来源: SciELO

【 摘 要 】

During some phases of of their life-cycle endophytic fungi colonize plants asymptomatically being found most frequently inside the aerial part of plant tissues. After surface disinfection of apparently healthy leaves from three varieties of mandarin orange and one tangor, and after incubation on appropriate culture medium, 407 fungal isolates were obtained, giving a total infection frequency of 81%. No fungal growth was observed from disinfected seeds, indicating that fungi are probably not transmitted via seeds. Of the fungal isolates, 27% belonged to the genus Guignardia, with 12 isolates being identified as Guignardia citricarpa Kiely, which is described as a citrus pathogen. The isolates were variable in respect to the presence of sexual structures and growth rates. Most of the isolates produces mature asci, supporting the hypothesis that they are nonpathogenic endophytes, which recently were identified as G. mangiferae. High intraspecific genetic variability (an average similarity coefficient of 0.6) was detected using random amplified polymorphic DNA (RAPD) markers generated by seven different primers. The highest similarity coefficient (0.9) was between isolates P15 and M86 and the smallest (0.22) between isolates P15 and C145. These results did not allow us to establish an association between genetic similarity of the fungal isolates and the citrus varieties from which they were obtained.

【 授权许可】

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