Ex-vivo Effect of Intracanal Medications Based on Ozone and Calcium Hydroxide in Root Canals Contaminated with Enterococcus faecalis

Brazilian Dental Journal

Roberta Vieira Farac¹  Antonio Carlos Pizzolitto¹  Juliane Maria Guerreiro Tanomaru¹  Renata Dornelles Morgental¹  Regina Karla De Pontes Lima¹  Idomeo Bonetti-filho¹ 

关键词: calcium hydroxide;    Endodontics;    Enterococcus faecalis;    ozone;    root canal preparation;   

DOI  :  10.1590/0103-6440201301992

来源: SciELO

【 摘 要 】

This ex vivo study evaluated the antibacterial effect of intracanal medications in root canals contaminated with Enterococcus faecalis. Fifty single-rooted human teeth were contaminated with E. faecalis (ATCC 29212) and incubated at 37°C for 21 days. The specimens were randomly divided into 5 groups according to the intracanal medication used: OZ-PG: ozonized propylene glycol; CH/CPMC: calcium hydroxide/camphorated paramonochlorophenol; OZ-PG/CH ozonized PG/CH; PC: positive control group (no medication); and NC: negative control group (no contamination). The samples were collected after 7 days (post-medication) and 14 days (final). Bacterial growth was checked by counting the colony-forming units (CFU). OZ-PG and CH/CPMC reduced significantly the CFU counts compared with PC in the post-medication and final samples, with no statistically significant differences among them. On the other hand, OZ-PG/CH did not reduce significantly the number of bacteria compared with PC. In conclusion, among the evaluated medications OZ-PG and CH/CPMC were the most effective against E. faecalis.

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