Detection of streptococcus mutans and streptococcus sobrinus in dental plaque samples from Brazilian preschool children by polymerase chain reaction

Brazilian Dental Journal

Teresa Cristina Costa Franco E Franco²  Patrícia Amoroso²  José Moacir Marin¹  Fernando Antonio De Ávila¹ 

[1] ,Educational Foundation of Barretos School of Dentistry Barretos SP ,Brazil

关键词: mutans streptococci;    dental caries;    preschool children;    polymerase chain reaction;   

DOI  :  10.1590/S0103-64402007000400011

来源: SciELO

【 摘 要 】

The purposes of this study were to detect S. mutans and S. sobrinus by polymerase chain reaction (PCR) amplification, and to relate their presence to the incidence of dental caries in 42 Brazilian preschool children. Dental plaque samples were collected from the cervical margin of all erupted teeth of 5-6 years old children with primary dentition, using a sterile explorer. Examination of the dmft (decayed, missing, filled teeth) index, performed following the World Health Organization (WHO) caries diagnostic criteria, showed a 2.71 score. Prevalence of S. mutans and S. sobrinus was respectively, of 85.7% and 14.3%; no dental plaque sample was either positive or negative for both bacterial species. Children harboring either S. mutans or S. sobrinus presented the same caries prevalence. PCR showed good discriminative ability for differentiation between these species, and suggested that it is a technique suitable for epidemiological studies on mutans streptococci.

【 授权许可】

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