Brazilian Dental Journal | |
Biofilms of black tooth stains: PCR analysis reveals presence of Streptococcus mutans | |
Marília Teixeira Costa1  Miriam Leandro Dorta1  Fátima Ribeiro-dias1  Fabiana Cristina Pimenta1  | |
[1] ,Federal University of Goiás Institute of Tropical Pathology and Public Health Goiânia GO ,Brazil | |
关键词: black tooth stain; dental caries; mutans streptococci; black-pigmented bacteria; PCR; | |
DOI : 10.1590/S0103-64402012000500014 | |
来源: SciELO | |
【 摘 要 】
This study investigated the presence of the black-pigmented bacteria Prevotella nigrescens and Prevotella intermedia, the non-black-pigmented bacteria Actinomyces spp and particularly the cariogenic pathogen Streptococcus mutans in the dental biofilms of patients with or without black extrinsic tooth stains, using the multiplex polymerase chain reaction (PCR) technique. Analysis of the dental biofilms of patients with (n=26) or without (n=26) black tooth stains was performed using duplex PCR for the 16S ribosomal RNA gene (P. nigrescens, P. intermedia, Actinomyces spp) and glucosyltransferase-I gene for S. mutans. P. nigrescens and S. mutans were the most frequent bacteria detected in both groups. The least frequently detected were P. intermedia and Actinomyces spp. The similar bacterial composition of dental biofilms of black tooth stains and healthy tooth surfaces indicates that black tooth stains are not free of cariogenic bacteria.
【 授权许可】
CC BY
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
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RO202005130109845ZK.pdf | 387KB | download |