Transmission of Aggregatibacter actinomycetemcomitans between Brazilian women with severe chronic periodontitis and their children

Brazilian Dental Journal

Rodrigo Otávio Citó César Rêgo²  Denise Madalena Palomari Spolidorio¹  Sérgio Luiz De Souza Salvador¹  Joni Augusto Cirelli¹ 

[1] ,Federal University of Ceará School of Pharmacy, Dentistry and Nursing Department of Clinical DentistryFortaleza CE ,Brazil

关键词: periodontal diseases;    microbiology;    Aggregatibacter actinomycetemcomitans;    periodontal diseases;    transmission;    families;   

DOI  :  10.1590/S0103-64402007000300008

来源: SciELO

【 摘 要 】

This study evaluated the transmission of Aggregatibacter actinomycetemcomitans (Aa) in women with severe chronic periodontitis and their children. Thirty women (mean age = 36.1±6.0 years) who were mothers of at least one child aged 7 to 16 years were enrolled. In order to investigate mother-child transmission of Aa, the children were also evaluated when their mothers were colonized by the bacterium. Subgingival plaque samples of each woman were collected from 3 sites (mean probing depth of 7.3±1.2 mm and mean clinical attachment level of 7.9±1.5 mm) and pooled in reduced transport fluid (RTF). These samples were processed, inoculated onto TSBV-agar selective medium and incubated at 37°C in microaerophilic atmosphere for 5 days. Aa was identified on the basis of colony morphology, Gram staining, catalase and oxidase reactions. Aa was found in 8 out of 30 women. Therefore, 8 children from these women (mean age= 12 ± 3.7 years) were evaluated, but Aa was found only in 2 of them. Aa strains of the two mother-child pairs were evaluated by arbitrarily-primed polymerase chain reaction (AP-PCR), although it was not found similarity between the amplitypes of each pair. No Aa transmission was found between Brazilian women with severe chronic periodontitis and their children.

【 授权许可】

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