Expression and Activity of Matrix Metalloproteinase-2 (MMP-2) in the development of rat first molar tooth germ

Brazilian Dental Journal

Paola Cotrim²  Cleverton Roberto De Andrade²  Sergio Line¹  Oslei Paes De Almeida²  Ricardo D. Coletta² 

[1] ,UNICAMP Faculty of Dentistry of Piracicaba Discipline of Oral Patology

关键词: matrix metalloproteinase-2;    tooth germ;    rat;    metaloproteinase de matriz-2;    germe dental;    rato;   

DOI  :  10.1590/S0103-64402002000200004

来源: SciELO

【 摘 要 】

Tooth germ development is associated with morphological and biochemical changes of the dental papilla and enamel organ. Enzymes with gelatinolytic activities were studied by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and enzymography in tooth germ of newborn to 15-day-old rats. Three major bands with gelatinolytic activity were detected at all periods and characterized as the latent and active forms of MMP-2 using their molecular weight and activity dependent on Zn++ and Ca++ ions as criteria. Expression and activity of MMP-2 increased progressively from 0 to 15 days after birth. Mechanical separation of the tooth germ from 10-day-old rats showed that the gelatinolytic activity was localized mainly in the dental papilla and not the dental organ. These data indicate that the expression and activity of MMP-2 varies during the development and maturation of rat first molar tooth germ.

【 授权许可】

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