Facile chemiluminescence assay for acyl-CoA oxidase activity: fundamentals and illustrative examples

Journal of the Brazilian Chemical Society

Min Yao²  Xiangdong Xu¹  Yang Liu²  Lingling Jiang² 

[1] ,Hebei Medical University School of Public Health China Administration of Education,China

关键词: acyl-CoA oxidase;    chemiluminescence;    luminol;    peroxisomal oxidation;   

DOI  :  10.5935/0103-5053.20140039

来源: SciELO

【 摘 要 】

Acyl-CoA oxidase (ACO) is the key enzyme that catalyzes the rate-determining step in the peroxisomal beta-oxidation of fatty acids. ACO catalyzes the oxidation of acyl-CoA with molecular oxygen to produce trans-2,3-dehydroacyl-CoA and H2O2. Given the participation of H2O2 in an enhanced chemiluminescent reaction with luminol, we have developed a novel "two-step" procedure for the quantitation of ACO activity by recording light emission using a chemiluminescence detector. A number of recommendations on standardizing the reaction conditions for the quantitative measurement of ACO-catalyzed reactions are offered. The proposed method is simple and reliable and has been successfully applied in the rat liver assay. The results indicate that ACO activities increased in the liver of type-2 diabetic rats but showed no significant change in the liver of insulin-resistant rat.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License