Facile chemiluminescence assay for acyl-CoA oxidase activity: fundamentals and illustrative examples

Journal of the Brazilian Chemical Society

Jiang, Lingling¹  Hebei Medical University, China¹  Xu, Xiangdong¹  Liu, Yang¹  Yao, Min¹ 

关键词: acyl-CoA oxidase;    chemiluminescence;    luminol;    peroxisomal oxidation.;   

DOI  :  10.5935/0103-5053.20140039

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

One of the keys to maintain high manufacturing quality improvement is the use of control charts. In this work, multivariate control charts based on significant principal component (PC) scores and net analytical signal (NAS) were developed to simultaneously monitor the quality of two active pharmaceutical ingredients (API) (isoniazid and rifampicin) in pharmaceutical formulation (laboratory samples and production samples) using a portable near infrared spectrometer. The limits for both multivariate charts were estimated using the quality specifications from the pharmaceutical formulation (± 5% of the nominal content of each API). The use of these multivariate control charts has provided a simple and powerful tool to evaluate the content of pharmaceutical formulations based on isoniazid and rifampicin capsules combined with near infrared spectroscopy. The procedure is rapid and adjustable for monitoring the production of the pharmaceutical preparations produced at UFRN/Brazil toward the process analytical technology (PAT) for the treatment of pulmonary tuberculosis.

【 授权许可】

Unknown