Potent antileukemic action of naphthoquinoidal compounds: evidence for an intrinsic death mechanism based on oxidative stress and inhibition of DNA repair

Journal of the Brazilian Chemical Society

Bruno C. Cavalcanti²  Igor O. Cabral²  Felipe A. R. Rodrigues²  Francisco W. A. Barros²  Danilo D. Rocha²  Hemerson I. F. Magalhães²  Dinara J. Moura¹  Jenifer Saffi¹  João A. P. Henriques¹  Tatiane S. C. Carvalho¹  Manoel O. Moraes²  Cláudia Pessoa²  Isadora M. M. De Melo¹  Eufrânio N. Da Silva Júnior¹ 

[1] ,Federal University of Ceará Department of Physiology and Pharmacology National Laboratory of Experimental OncologyFortaleza CE ,Brazil

关键词: naphthoquinone;    nor-β-lapachone;    antileukemic activity;    reactive oxygen species;    genotoxicity;    DNA repair;   

DOI  :  10.1590/S0103-50532013000100019

来源: SciELO

【 摘 要 】

The current study describes that nor-β-lapachone and its arylamino derivatives, iodinated and methylated naphthoquinones and nor-β-lapachone-based 1,2,3-triazoles exhibited pronounced cytotoxic effects against four human leukemia cell lines (HL-60, K562, Molt-4 and Jurkat). Nor-β-lapachones arylamino substituted with potent activity were identified, revealing themselves as potential prototypes against tumor cell lines. Moreover, cells treated with these compounds showed DNA damage according to the standard comet assay, a finding that was, at least in part, due to increased intracellular levels of ROS. HL-60 cells were chosen to study the underlying molecular mechanisms of cytotoxicity. Drug-induced apoptosis in HL-60 cells was observed by flow cytometry analyses. Strains of Saccharomyces cerevisiae were used for a preliminary investigation into the mechanism of drug action on DNA topoisomerases. These results suggested that the cytotoxicity of these compounds apparently does not involve topoisomerase inhibition, but that treatment impairs DNA repair activity, thus triggering cell death. Considering their pro-oxidant properties, we investigated the ability of these compounds to induce apoptosis and chromosomal aberrations as micronuclei in Chinese hamster lung fibroblasts (V79 cells). Morphological apoptotic nuclei and micronuclei induction following drug treatment were observed, suggesting a correlation between DNA damage and apoptosis.

【 授权许可】

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