Selective and efficient mitochondrial staining with designed 2,1,3-benzothiadiazole derivatives as live cell fluorescence imaging probes

Journal of the Brazilian Chemical Society

Brenno A. D. Neto¹  José R. Corrêa¹  Pedro H. P. R. Carvalho¹  Diego C. B. D. Santos¹  Bruna C. Guido¹  Claudia C. Gatto¹  Heibbe C. B. De Oliveira¹  Maíra Fasciotti²  Marcos N Eberlin²  Eufrânio N. Da Silva Jr.² 

[1],University of Brasília Chemistry Institute Laboratory of Medicinal and Technological ChemistryBrasília DF ,Brazil

关键词: benzothiadiazole;    synthesis;    mitochondria;    cellular staining;   

DOI  :  10.1590/S0103-50532012000400024

来源: SciELO

【 摘 要 】

Novel designed 2,1,3-benzothiadiazole fluorescent probes were synthesized, characterized and applied as live cell fluorescence imaging probe staining only mitochondria in mammalian cancer cell lines (MCF-7). The efficiency of these new probes was found to be much superior to that of the commercially available MitoTracker® Red. Cellular and in vitro experiments allowed better understanding of the relationship between the planned molecular architecture of the new dyes and the observed cellular selectivity.

【 授权许可】

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