Selective and efficient mitochondrial staining with designed 2,1,3-benzothiadiazole derivatives as live cell fluorescence imaging probes

Journal of the Brazilian Chemical Society

D. Neto, Brenno A.¹  Eberlin, Marcos N¹  Gatto, Claudia C.¹  Corrêa, José R.¹  Fasciotti, Maíra¹  University of Brasília, Brasília, Brazil¹  Federal University of Minas Gerais, Belo Horizonte, Brazil¹  Santos, Diego C. B. D.¹  Guido, Bruna C.¹  Carvalho, Pedro H. P. R.¹  University of Campinas, Campinas, Brazil¹  Oliveira, Heibbe C. B. de¹  Silva Jr., Eufrânio N. da¹ 

关键词: benzothiadiazole;    synthesis;    mitochondria;    cellular staining;   

DOI  :  10.1590/S0103-50532012000400024

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

Novel designed 2,1,3-benzothiadiazole fluorescent probes were synthesized, characterized and applied as live cell fluorescence imaging probe staining only mitochondria in mammalian cancer cell lines (MCF-7). The efficiency of these new probes was found to be much superior to that of the commercially available MitoTracker® Red. Cellular and in vitro experiments allowed better understanding of the relationship between the planned molecular architecture of the new dyes and the observed cellular selectivity.

【 授权许可】

Unknown