Systematic UPLC-ESI-MS/MS study on the occurrence of staurosporine and derivatives in associated marine microorganisms from Eudistoma vannamei

Journal of the Brazilian Chemical Society

Márcio A. Andréo¹  Paula C. Jimenez²  Josy B. C. N. Siebra²  Leticia V. Costa-lotufo²  Ricardo Vessecchi¹  Michael Niehues¹  João L. C. Lopes¹  Norberto P. Lopes¹ 

[1] ,Universidade de São Paulo Faculdade de Ciências Farmacêuticas de Ribeirão Preto Núcleo de Pesquisa em Produtos Naturais e Sintéticos (NPPNS)Ribeirão Preto SP ,Brazil

关键词: Eudistoma vannamei;    Streptomyces;    staurosporine;    LC-MS;    ascidian;   

DOI  :  10.1590/S0103-50532012000200021

来源: SciELO

【 摘 要 】

Former bioactivity-guided analysis of the marine invertebrate Eudistoma vannamei led to the isolation of staurosporine derivatives, which revealed strong cytotoxic activity against several human cancer cell lines. The occurrence of such alkaloids in E. vannamei may be correlated to the presence of associated biota, such as Streptomyces bacteria. In agreement to this hypothesis, marine microorganisms associated with E. vannamei were recovered and cultured, leading to a total of 84 isolated bacterial strains. Gas phase fragmentation reactions of staurosporine and derivatives were systematically studied and the analyzed results further supported by computational chemistry studies. The resulting fragment patterns were used to search for the presence of different derivatives in extracts of isolated microorganisms, thereby using LC-MS/MS analysis in MRM mode. These results evidenced that one isolated Streptomyces sp. was able to generate staurosporine, while none of the hydroxy-7-oxo derivatives were detected. Finally, significant cytotoxic activity against human cancer lines was observed for one of the extracts.

【 授权许可】

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