Systematic UPLC-ESI-MS/MS study on the occurrence of staurosporine and derivatives in associated marine microorganisms from Eudistoma vannamei

Journal of the Brazilian Chemical Society

Lopes, João L. C.¹  Universidade de São Paulo, Ribeirão Preto, Brazil¹  Niehues, Michael¹  Andréo, Márcio A.¹  Universidade Federal do Ceará, Fortaleza, Brazil¹  Siebra, Josy B. C. N.¹  Vessecchi, Ricardo¹  Costa-Lotufo, Leticia V.¹  Jimenez, Paula C.¹  Lopes, Norberto P.¹ 

关键词: Eudistoma vannamei;    Streptomyces;    staurosporine;    LC-MS/MS;    ascidian;   

DOI  :  10.1590/S0103-50532012000200021

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

Former bioactivity-guided analysis of the marine invertebrate Eudistoma vannamei led to the isolation of staurosporine derivatives, which revealed strong cytotoxic activity against several human cancer cell lines. The occurrence of such alkaloids in E. vannamei may be correlated to the presence of associated biota, such as Streptomyces bacteria. In agreement to this hypothesis, marine microorganisms associated with E. vannamei were recovered and cultured, leading to a total of 84 isolated bacterial strains. Gas phase fragmentation reactions of staurosporine and derivatives were systematically studied and the analyzed results further supported by computational chemistry studies. The resulting fragment patterns were used to search for the presence of different derivatives in extracts of isolated microorganisms, thereby using LC-MS/MS analysis in MRM mode. These results evidenced that one isolated Streptomyces sp. was able to generate staurosporine, while none of the hydroxy-7-oxo derivatives were detected. Finally, significant cytotoxic activity against human cancer lines was observed for one of the extracts.

【 授权许可】

Unknown