Determination of pesticide residues in sugarcane honey by QuEChERS, and liquid chromatography

Journal of the Brazilian Chemical Society

Maicon R. F. Sampaio¹  Débora Tomasini¹  Liziane V. Cardoso¹  Sergiane S. Caldas¹  Ednei G. Primel¹ 

[1] ,Universidade Federal do Rio Grande Escola de Química e Alimentos Rio Grande RS ,Brazil

关键词: pesticides;    sugarcane honey;    QuEChERS;    liquid chromatography;   

DOI  :  10.1590/S0103-50532012000200002

来源: SciELO

【 摘 要 】

This paper aims at describing a method to determine the pesticides 2,4-D, diuron and fipronil in sugarcane honey. The QuEChERS method was employed for extraction and the quantification was carried out by high performance liquid chromatography coupled with diode array detector (HPLC-DAD) and liquid chromatography-tandem mass spectrometry with electrospray ionization source (LC-ESI-MS/MS). After the optimization of the extraction parameters and the determination of the pesticides, the method was validated by evaluating the analytical curve, linearity, limits of detection and quantification, precision (repeatability) and accuracy (recovery). Due to the occurrence of matrix effect, the quantification was performed using the analytical standards prepared in the blank matrix extract. The method provided limits of detection in the range of 0.016-0.1 mg kg-1 by HPLC-DAD and 0.00016-0.0008 mg kg-1 by LC-ESI-MS/MS. The method was applied to the determination of pesticides in sugarcane honey.

【 授权许可】

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