期刊论文详细信息
Journal of the Brazilian Chemical Society
Enzymatic synthesis optimization of isoamyl butyrate
Andréia Anschau1  Vitor C. Aragão1  Barbara D. A. Porciuncula1  Susana J. Kalil1  Carlos A. V. Burkert1  Janaína F. M. Burkert1 
[1] ,Universidade Federal do Rio Grande Escola de Química e Alimentos Rio Grande RS ,Brazil
关键词: flavor ester;    Lipozyme TL-IM;    organic media;    response surface methodology (RSM);    fusel oil;   
DOI  :  10.1590/S0103-50532011001100018
来源: SciELO
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【 摘 要 】

Lipozyme TL IM was used to catalyse the esterification of isoamyl alcohol and butyric acid. A fractional factorial design was employed to evaluate the effects of temperature (30, 40, 50 °C), alcohol:acid molar ratio (1:1, 2:1, 3:1), enzyme concentration (0.003, 0.0115, 0.020 g mL-1), butyric acid concentration (0.1, 0.3, 0.5 mol L-1) and shaking rate (50, 115, 180 rpm) on the ester yield. With these results, the levels were redefined to a 2³ factorial design. The maximum yield of ester was obtained at 30 ºC, 180 rpm, alcohol:acid molar ratio of 1:1, enzyme concentration of 0.021 g mL-1 and butyric acid concentration of 0.5 mol L-1. Under the optimal conditions, 92% esterification was attained with an ester concentration of 0.9 mol L-1. Isoamyl alcohol from fusel oil was used under the same conditions and resulted in 93% esterification and an ester concentration of 1.0 mol L-1.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License

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