STD NMR spectroscopy: a case study of fosfomycin binding interactions in living bacterial cells

Journal of the Brazilian Chemical Society

Cíntia D. F Milagre¹  Luís Fernando Cabeça¹  Lucas G Martins¹  Anita J Marsaioli¹ 

[1] ,Universidade Estadual de Campinas Instituto de Química Campinas SP ,Brazil

关键词: STD NMR in living bacterial cells;    membrane-bound proteins;    ligand-target interactions;    fosfomycin;    liposomes;   

DOI  :  10.1590/S0103-50532011000200014

来源: SciELO

【 摘 要 】

A saturation transfer difference (STD) NMR experiment was successfully employed to observe the binding interactions of fosfomycin resistant and non-resistant bacterial strains using living cell suspensions, without the need for isotopic labelling of the ligand or receptor.

【 授权许可】

CC BY   
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