A kinetic study of candida rugosa lipase-catalyzed synthesis of 4,6-dimethyl-3-cyano-2-pyridone

Journal of the Brazilian Chemical Society

Nevena Ž Prlainović¹  Dejan I. Bezbradica¹  Zorica D. Knežević-jugović¹  Roksana T. Kozlowska¹  Dušan Ž. Mijin¹ 

[1] ,Faculty of Technology and MetallurgyBelgrade,Serbia

关键词: Candida rugosa lipase;    ping-pong kinetics;    cyanoacetamide inhibition;    acetylacetone;    pyridon;   

DOI  :  10.1590/S0103-50532010001200016

来源: SciELO

【 摘 要 】

The synthesis of 4,6-dimethyl-3-cyano-2-pyridone was studied in a reaction catalyzed with Candida rugosa lipase. A response surface methodology (RSM) was applied for the optimization of the experimental conditions. The study showed that temperature and molar ratio cyanoacetamide/acetylacetone have a great influence on the yield of the reaction; the maximum yield was achieved at 44 ºC with a molar ratio cyanoacetamide/acetylacetone of 36. The kinetics of the enzymatic synthesis of 4,6-dimethyl-3-cyano-2-pyridone was investigated, and it was determined that a ping-pong bi-ter mechanism with cyanoacetamide inhibition fits the obtained results. The mechanism of lipase-catalyzed reaction was proposed.

【 授权许可】

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