Journal of the Brazilian Chemical Society | |
A kinetic study of candida rugosa lipase-catalyzed synthesis of 4,6-dimethyl-3-cyano-2-pyridone | |
Faculty of Technology and Metallurgy, Belgrade, Serbia1  Prlainović, Nevena Ž1  Mijin, Dušan Ž.1  Knežević-Jugović, Zorica D.1  Bezbradica, Dejan I.1  Kozlowska, Roksana T.1  | |
关键词: Candida rugosa lipase; ping-pong kinetics; cyanoacetamide inhibition; acetylacetone; pyridone; | |
DOI : 10.1590/S0103-50532010001200016 | |
学科分类:化学(综合) | |
来源: SciELO | |
【 摘 要 】
The synthesis of 4,6-dimethyl-3-cyano-2-pyridone was studied in a reaction catalyzed with Candida rugosa lipase. A response surface methodology (RSM) was applied for the optimization of the experimental conditions. The study showed that temperature and molar ratio cyanoacetamide/acetylacetone have a great influence on the yield of the reaction; the maximum yield was achieved at 44 ºC with a molar ratio cyanoacetamide/acetylacetone of 36. The kinetics of the enzymatic synthesis of 4,6-dimethyl-3-cyano-2-pyridone was investigated, and it was determined that a ping-pong bi-ter mechanism with cyanoacetamide inhibition fits the obtained results. The mechanism of lipase-catalyzed reaction was proposed.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912050580903ZK.pdf | 873KB | download |