Exploiting iminoquinone free radical production for thiol based drugs determination in pharmaceutical formulations

Journal of the Brazilian Chemical Society

Manoel P. Chagas²  Josué C. C. Santos²  Eduardo B. G. N. Santos¹  Tiago D. Oliveira¹  Mauro Korn¹ 

[1] ,Universidade Federal da Bahia Instituto de Química Salvador BA ,Brazil

关键词: iminoquinone radical reaction;    flow injection analysis;    determination of thiol drugs;    Captopril;    N-acetyl-L-cysteine;   

DOI  :  10.1590/S0103-50532009000900012

来源: SciELO

【 摘 要 】

A spectrophotometric flow analysis procedure to determine Captopril (CPT) and N-acetyl-L-cysteine (NAC) in pharmaceutical formulations was developed based on the reaction with iminoquinone radical produced from the N,N-dimethyl-p-phenilenediamene (DMPD) oxidation in acid medium. The reagents addition order was evaluated and analytical parameters were optimized. The linear concentrations ranges were 2.5-90 and 5.0-50 mg L-1 for CPT and NAC, respectively. Limits of detection (3σ) were calculated at 0.22 and 1.8 mg L-1 and the relative standard deviation (N = 10) were lower than 1.0 and 2.1% for CPT and NAC, respectively. The procedure was employed for CPT and NAC determination in diverse pharmaceutical formulations and by applying paired t-test the results were in agreement with those obtained by reference methods for 95% confidence level.

【 授权许可】

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